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KMID : 0380019940090030339
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Korean Journal of Biotechnology and Bioengineering
1994 Volume.9 No. 3 p.339 ~ p.344
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Effect of Glucose Repression and Plasmid Copy Number on Cloned Gene Expression in Recombinant Yeast
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Hong Eock-Kee
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Abstract
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Deletions between UASG and the GALL TATA box reduced glucose repression and allowed constitutive expression of the gene product in the absence of galactose. The relative inducer level (ratio of galactose/glucose concentrations) affected the extent of gene expression and glucose repression. Glucose repression was reduced by a factor of 2 to 5 as the relative inducer level increased. In the medium containing galactose only, induction of /3-galactosidase synthesis by galactose increased with plasmid copy number. On the contrary, plasmid copy number didnot affect significantly Q galactosidase synthesis in the medium containing both glucose and galactose (2% glucose+2% galactose), which might be due to glucose repression caused by high glucose concentraion. Howeve;when the medium contained the relatively high inducer level (0.4% glucose-0.8% galactose). - galactosidase synthesis increased with plasmid copy numbe;indicating that the beneficial effect of higher galactose concentration was weaker than the repressive effect of higher glucose concentration.
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KEYWORD
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